Constructing and expressing acyl-homoserine lactone lactonase (aiiA) gene for enhancing Solanum tuberosum resistance against soft rot disease
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Abstract. Suryanti E, Rusamana I, Wahyudi AT, Akhdiya A, Sukamdjaja D. 2022. Constructing and expressing acyl-homoserine lactone lactonase (aiiA) gene for enhancing Solanum tuberosum resistance against soft rot disease. Biodiversitas 23: 1780-1787. Expression of the genes associated with pectinase production in soft rot disease of Dickeya spp. are regulated by quorum sensing mechanism using acyl homoserine lactone(AHL). Degradation of AHL using AHL lactonase can be developed to reduce pectinase production in Dickeyaspp.The aiiA gene encodes AHL lactonase from Bacillus spp. This study aims to construct binary vector of inserted aiiA gene (pCAB10) from Bacillus cereus B10 and express the aiiA gene in potato Medians cultivar genome. The aiiAgene was cloned into binary vector and transformed into Agrobacterium tumefaciens LB4404. The Agrobacterium-mediated genetic transformation was conducted with co-cultivation technique.The transgenic plant was confirmed through PCR andselected through kanamycin and soft rot disease resistance to Dickeya dadantii A3. The result showed that aiiA gene was successfully integrated top CAB10 and introduced to A. tumefaciens LB4404. The percentage of pCAB10 transformation was 57.6%, while there generation efficiency was 11.9% with 50 mg/L acetosyringone addition. Four transgenic potato Medians cultivar exhibited significantly higher resistance against soft rot disease than non-transgenic plants. Mahl1 clone had the highest resistance to D. dadantii with soft rot disease incidence as 7.1% and percentage of soft rot disease suppression as 92.85%.
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References
Ban H, Chai X, Lin Y, Zhou Y, Peng D, Zhou Y, Zou Y, Yu Z, Sun M. 2016. Transgenic Amorphophallus konjac expressing synthesized acyl-homoserine lactonase (aiiA) gene exhibit enhanced resistance to soft rot disease. Plant Cell Reports 28 : 1847-1855. https://doi.org/10.1007/s00299-009-0788-x
Crepin A, Cirou AB, Barbey C, Farmer C, Hélias V, Burini JF, Faure D, Latour X. 2012. N-acyl homoserin lactones in diverse Pectobacterium and Dickeya plant pathogen: diversity, abudance and involvement in virulence. Sensor 12 (3) : 3494-3497. https://doi.org/10.3390/s120303484
Chen F, Gao Y, Chen X, Yu Z, Li X. 2013. Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. International Journall of Molecular Sciences 14 : 17477-17500. https://doi.org/10.3390/ijms140917477
Degefu Y, Potrykus M, Golanowska M, Virtanen E, Lojkowska E. 2013. A new clade of Dickeya spp. plays a major role in potato blackleg outbreaks in North Finland. Annals of Applied Biology 162 : 231-241. https://doi.org/10.1111/aab.12020
Dong YH, Xu JL, Li XC, Zang LH. 2000. aiiA, a novel enzyme inactivates acyl homoserine-lactone quorum sensing signal and attenuates the virulence of Erwinia caratovora. Proceedings of the National Academy of Sciences 97: 3526-3531. https://doi.org/10.1073/pnas.97.7.3526
Dong YH, Wang LH, Xu LJ, Zhang HB, Zhang XF, Zhang LH. 2001. Quenching quorum-sensing dependent bacterial infection by an N-acyl homoserine lactonase. Nature 41 : 813-817. https://doi.org/10.1038/35081101
Fan X, Ye T, Li Q, Bhatt P, Zhang L, Chen S. 2020. Potential of a quorum quenching bacteria isolate Ochrobactrum intermedium D-2 against soft rot pathogen Pectobacterium carotovorum subsp. carotovorum. Frontiers in Microbiology 11: 898,1-14. https://doi.org/10.33 89/fmicb.2020.00898
Garge SS, Nerurkar AS. 2017. Evaluation of quorum quenching Bacillus spp. for their biocontrol traits against Pectobacterium carotovorum subsp. carotovorum causing soft rot. Biocatalysis and Agricultural Biotechnology 9 : 48–57. https://doi.org/10.1016/j.bcab.2016.11.004
Gea N, Suharsono G, Wattimena A, Widyastuti U. 2017. Introduction of Hd3a gene in IPB CP1 potato cultivar through Agrobacterium tumefaciens-mediated transformation under the control of use 35S CaMV promoter. Pakistan Journal of Biotechnology 14(2): 129-134.
Han LW., Yao L, Ge Y, Cui CS. 2015. Transformation of soft rot resistant aiiA gene into Chinese Cabbage (Brassica rapa ssp. pekinensis) by the pollen-tube pathway.International Journal of Agriculture and Biology 17(3): 668-670. https://doi.org/10.17957/IJAB/17.3.14.265
Handayani, T., Alimuddin., Widyastuti, U., Suryati, E., and Parenrengi, A. (2014). Binary vector construction and-mediated transformation of lysozyme gene in Seaweed Agrobacterium tumefaciens Kappaphycus alvarezii. Biotropia 21(2): 80-90.
Hosseinzadeh S, Masoud SB, Majid S. 2016. Attenuation and quantitation of virulence gene expression in quorum-quenched Dickeya chrysanthemi. Archives of Microbiology, 199, Issue 1, 51-61. https://doi.org/10.1007/s00203-016-1276-7
Huma N, Shankar P, Kushwah J, Bhushan A, Joshi J, Mukherjee T, Raju S, Purohit HJ, Kalia VC. 2011. Diversity and polymorphism in AHL-lactonase gene (aiiA) of Bacillus. Journal Microbiology and Biotechnology 21: 1001-1011.https://doi.org/10.4014/jmb.1105.05056
Ismiyatuningsih, Joko T, Hartono S. 2016. Survey and detection of Pectobacterium atrosepticum in major potato-growing areas in Central Java province, Indonesia. Agricultural Science 1(1): 001-006. https://doi.org/10.22146/ipas.11654
Kado CI. 2014. Historical account ongaining insights on the mechanism of crown gall tumorigenesis induced by Agrobacterium tumefaciens. Frontier in Microbiology 5(340): 1-15. https://doi.org/0.3389/fmicb.2014.00340.
Khoiri S, Damayanti TS, Giyanto. 2017. Identification of quorum quenching bacteria and its biocontrol potential against soft rot disease bacteria, Dickeya dadantii. AGRIVITA Journal of Agricultural Science 39(1): 45-55. https://doi.org/10.17503/agrivita.v39i1.633
Krzyzanowska DM, Potrykus M, Golanowska M, Polonis K, Wisniewska AG, Lojkowska E, Jafra S. 2012. Rhizosphere bacteria as potential biocontrol agents against soft rot caused by various Pectobacterium and Dickeya spp. strains. Journal of Plant Pathology 94,(2): 367-378
Kumar V, Sharma A, Prasad BCN, Gururaj HB, Ravishankar GA. 2006. Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment. Electronic Journal Of Biotechnology 9(4): 349-356 https://doi.org/ 10.2225/vol9-issue4-fulltext-4.
Mayavan S, Subramanyam K, Arun M, Rajesh M, Dev GK, Sivanandhan G, Jaganath B, Manickavasagam M, Selvaraj N. 2013. Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane. Plant Cell Reports 32(10): 1557-1574. https://doi.org/0.1007/s00299-013-1467-5
Murai N. 2013. Review: plant binary vectors of ti plasmid in Agrobacterium tumefaciens with a broad host-range replicon of pRK2, pRi, pSa or pVS1. American Journal of Plant Sciences 4: 932-939. http://dx.doi.org/10.4236/ajps.2013.44115
Nickoloff JA. 1995. Methods in molecular biology, vol 37.Totowa, NJ: Humana Press
Ouyang L, Li M. 2016. Effects of an inducible aiiA gene on disease resistance in Eucalyptus urophylla X Eucalyptus grandis. Transgenic Research 25(4): 441-452.https://doi.org/1007/s11248-016-9940-x.
P?curar DI, Thordal-Christensen H, P?curar ML, Pamfil D, Botez C, Bellini C. 2011. Agrobacterium tumefaciens: From crown gall tumors to genetic transformation. Physiological and Molecular Plant Pathology 76(2): 76–81. https://doi.org/0.1016/ j.pmpp.2011.06.004
Papenfort K, Bassle B. 2016. Quorum-sensing signal-response systems in gram-negative bacteria. Nature Review Microbiology 14(9): 576-588. https://doi.org /10.1038/nrmicro.2016.89.
Porto MS, Pinheiro MPN, Batista VGL, Santos RC, Filho AM, De Lima LM. 2014. Plant Promoters: an approach of structure and function. Molecular Biotechnology 56: 38–49.https://doi.org/10.1007/s12033-013-9713-1
Rashid H, Chaudhry Z, Khan MH. 2011. Effect of explant plant source and acetosyringone concentration on transformation efficiency of wheat cultivars. African Journal of Biotechnology 10(44): 8737-8740. https://doi.org/10.5897/AJB10.770
Reverchon S, Nasser W. 2013. Dickeya ecology, environment sensing and regulation of virulence programme. Environmental Microbiology Reports 5(5): 622-636. https://doi.org/0.1111/1758-2229.12073
Rosen R, Ron EZ. 2011. Proteomics of a plant pathogen: Agrobacterium tumefaciens. Proteomics 11(15): 3134–3142.https://doi.org /10.1002/pmic.201100019
Sambrook J, Russel DW. 2001. Molecular Cloning a Laboratory Manual. 3rd ed. New York,US : Cold Spring Harbour Laboratory Press
Sari PE, Rusmana I, Akhdiya A. 2016. AHL-lactonase characteristics of Bacillus thuringiensis SGT3g and its effectiveness in inhibiting pathogenicity of Dickeya dadantii. Malaysian Journal of Microbiology 12(4): 315-321. https://doi.org/10.21161/mjm.80515.
Sawant GB, Sawardekar SV, Bhave SG, Kshirsagar, JK. 2018. Effect of acetosyringone and age of callus on Agrobacterium-mediated transformation of rice (Oryza sativa L.) calli. International Journal of Chemistry 6(3): 82-88
Schuster M, Sexton DJ, Diggle SP, Greenberg EP. 2013. Acyl-homoserine lactone quorum sensing: from evolution to application. Annual Review of Microbiology 67: 43-63. https://doi.org/10.1146/annurev-micro-092412-155635
Talibi I, Askarne L, Boubaker H, Boudyach EH, Msanda F, Saadi B, Aoumar A. 2012. Antifungal activity of some Moroccan plants against Geotrichum candidum, the causal agent of postharvest citrus sour rot. Crop Protection 35: 41–46. https://doi.org/10.1016/j.cropro.2011.12.016
Toth IK, van der Wolf JM, Saddler G, Lojkowska E, Helias V, Pirhonen M, Tsror L, Elphinstone JG. 2011. Dickeya species: an emerging problem for potato production in Europe. Plant Pathology 60: 385-399. https://doi.org/10.1111/j.1365-3059.2011.02427.x
Tsror L, Erlich O, Lebiush S, Wolf JVD, Czajkowski R, Mozes G, Sikharulidze Z, Ben B. 2011. First report of potato blackleg caused by a biovar Dickeya sp. in Georgia. New Disease Reports 23, 1. https://doi.org/10.5197/j.2044-0588.2011.023.001
Zamani M, Behboudi K, Ahmadzadeh M. 2013. Quorum quenching by Bacillus cereus U92: a double-edged sword in biological control of plant diseases. Biocontrol Science and Technology 23(5) 555-573. https://dx.doi.org/10.1080/09583157.2013.787046
Zhang J, Feng T, Wang J, Wang Y, Zhang XH. 2019. The mechanisms and applications of Quorum Sensing (QS) and Quorum Quenching (QQ). Journal of Ocean University of China 18: 1427–1442. https://doi.org/10.1007/s11802-019-4073-5
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