Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia
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Abstract. Irawati W, Pinontoan R, Yuwono T. 2020. Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia. Biodiversitas 21: 5077-5084. Biological wastewater treatment using activated sludge is a promising wastewater treatment solution for removing heavy metals. To improve the effectiveness of biological wastewater treatment, activated sludge must consist of bacteria that can remove heavy metals through the process of bioaccumulation and biosorption. This study was aimed to isolate indigenous copper resistant bacteria and determining their resistance to copper, as well as analyzing their ability to accumulate and remove copper. Copper resistant bacteria were isolated from activated sludge of water treatment plant in industrial plant in Rungkut, Surabaya. Resistance to copper was analyzed by determining the value of minimum inhibitory concentration (MIC). The ability of bacterial isolates to remove copper was analyzed by atomic absorption spectrophotometer. A total of six highly copper resistant bacteria were isolated and designated as B6.1, C8.1, C9.3, C9.4, C9.5, C10.4 isolates. All isolates were categorized as high resistant bacteria with the MICs of 9-11 mM CuSO4. The two highest copper resistant bacteria were isolates C10.4 and C9.4. The ability of the two isolates to accumulate copper was 8.02 mg and 4.83 mg per gram dry weight of cells and to remove of copper up to 20.45% and 17.66%, respectively.
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References
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Sinha SN, Paul D. 2014. Heavy metal tolerance and accumulation by bacterial strains isolated from wastewater. J Chem Biol Phy Sci 4(1): 812-817.
Tortora GJ, Funke DR, Case CL. 2005. Microbiology: An Introduction. 8th ed. Benjamin Cummings, San Fransisco, CA.
Velkova Z, Stoytcheva M, Gochev V. 2012. Biosorption of Cu (II) onto chemically modified waste mycelium of Aspergillus awamori: equilibrium, kinetics and modeling studies. J Biosci Biotechnol 1(2): 163-169.
Xie X, Fu J, Wang H et al. 2010. Heavy metal resistance by two bacteria strains isolated from a copper mine tailing in China. Afr J Biotechnol 9(26): 4056-4066.
Zaidi A, Wani PA, Khan MS. 2012. Toxicity of Heavy Metals to Legumes and Bioremediation. Pondicherry, Springer
Adel AS, Gheethi A, Norli I et al. 2014. Biosorption of heavy metals and cephalexin from secondary effluents by tolerant bacteria. Clean Technol Environ Pol 16 (1): 137148.
Ahemad M. 2012. Implication of bacterial resistance against heavy metals in bioremediation: a review. J Inst Integrat Omics Appl Biotechnol 3 (3): 39-46.
Ahemad M, Malik A. 2011. Bioaccumulation of heavy metals by zinc resistant bacteria isolated from agricultural soils irrigated with wastewater. Bacteriol J 2(1): 12-21.
Al-Gheethi AA, Norli L, Lalung J et al. 2014. Biosorption of heavymetals and from secondary effluents by tolerant bacteria. Curr World Environ 16(1): 137-148.
Aljerf L, Almasri N. 2018. A gateway to metal resistance: bacterial response to heavy metal toxicity in the biological environment. Ann Adv Chem 2:32-4.
Altimera F, Yanez C, Bravo G et al. 2012. Characterization of copperresistant bacteria and bacterial communities from copper-polluted agricultural soils of central Chile. BMC Microbiol 12: 1-12.
Arisandi, P. 2013. The Rufford Small Grants for Nature Conservation. http://www.rufford.org/rsg/projects/prigi_arisandi
Benhalima L, Amri S, Bensouilah M et al. 2020. Heavy metal resistance and metallothionein induction in bacteria isolated from Seybouse river, Algeria. Appl Ecol Environ Res 18(1):1721-1737.
Chan KG, Atkinson S, Mathee K et al. 2011. Characterization of N-acylhomoserine lactonedegrading bacteria associated with Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia. BMC Microbiol. p11:51.
Chellaiah ER. 2018. Cadmium (heavy metals) bioremediation by Pseudomonas aeruginosa: a minireview. Appl Water Sci 8: 154.
Chen J, Li J, Zhang H et al. 2019. Bacterial heavy-metal and antibiotic resistance genes in a copper tailing dam area in Northern China. Front Microbiol 1-12.
Chipasa KB. 2003. Accumulation and fate of selected heavy metals in a biological wastewater treatment system. Waste Manage 23(2003): 135-143.
Gupta R, Kumar T, Mittal A. 2015. Isolation, identification and characterization of heavy metal resistant bacteria from soil of an iron industry, Haryana (India). Int J Pharm Sci Res 7(3): 1308-13.
Hansda A, Kumar V, Anshumali. 2015. Biosorption of copper by bacterial adsorbents: a review. Res J Environ Toxicol 9(2): 45-58.
Haq F, Butt M, Ali H et al. 2015. Biosorption of cadmium and chromium from water by endophytic Kocuria rhizophila: equilibrium and kinetic studies. Desalin Water Treat 57(42): 1-3.
Hobman JL, Crossman LC. 2014. Bacterial antimicrobial metal ion resistance. J Med Microbiol 64: 471–497.
Irawati W, Yuwono T, Rusli A. 2016. Detection of plasmids and curing analysis in Copper Resistant Bacteria Acinetobacter sp. C1, Acinetobacter sp. C2, and Cupriavidus sp. C4. Biodiversitas 17(1): 296-300.
Irawati W, Ompusunggu NP, Susilowati DN et al. 2019. Molecular and physiological characterization of indigenous copper-resistant bacteria from Cikapundung River, West Java, Indonesia. Biodiversitas 20(2): 344-349.
Kermani AJN, Ghasemi MF, Khosravan A et al. 2010. Cadmium bioremediation by metal-resistant mutated bacteria isolated from active sludge of industrial effluent. Iran. J Environ Health Sci Eng 7(4):279-286.
Komesli OT. 2014. Removal of heavy metals in wastewater by membrane bioreactor effects of flux and suction period. J Chem Soc Pakistan 36(4): 654-659.
Li C, Li Y, Ding C. 2019. The role of copper homeostasis at the host-pathogen axis: From bacteria to fungi. IJMS 20: 175.
Mahmoudkhani R, Torabian A, Hassani AH et al. 2014. Copper, cadmium and ferrous removal by membrane bioreactor. APCBEE 10: 79-83.
Manasi, Rajesh N, Rajesh V. 2016. Evaluation of the genetic basis of heavy metal resistance in an isolate from electronic industry effluent. J Genet Eng Biotechnol 14: 177-180.
Marzan LW, Hossain M, Mina SA et al. 2017. Isolation and biochemical characterization of heavy-metal resistant bacteria from tannery effluent in Chittagong city, Bangladesh: Bioremediation viewpoint. Egypt J Aquat Res 43: 65-74.
Murugavelh S, Vinothkumar D. 2010. Removal of heavy metals from waste water using different biosorbents. Curr World Environ 5(2): 299-304.
Nies DH. 1999. Microbial heavy-metal resistance. Appl Microbiol Biotechnol 51: 730–750.
Pipeline. 2003. Explaining the Activated Sludge Process. Spring 14(2): 1-8.
Rizvi A, Ahmed B, Zaidi A et al. 2019: Heavy metal mediated phytotoxic impact on winter wheat: oxidative stress and microbial management of toxicity by Bacillus subtilisBM2. RSC Advances 9: 6125-6142.
Rosahada AD, Budiyono, Dewanti NA. 2018. Biokonsentrasi logam berat tembaga (Cu) dan pola konsumsi ikan mujair di wilayah danau Rawapening. Jurnal Kesehatan Masyarakat 6(6):1-7.
Sari, SH, Kirana JF, Guntur. 2017. Analisis kandungan logam berat Hg dan Cu terlarut di Perairan Pesisir Wonorejo, Pantai Timur Surabaya. Jurnal Pendidikan Geografi 22(1), 1-9.
Sinha SN, Paul D. 2014. Heavy metal tolerance and accumulation by bacterial strains isolated from wastewater. J Chem Biol Phy Sci 4(1): 812-817.
Tortora GJ, Funke DR, Case CL. 2005. Microbiology: An Introduction. 8th ed. Benjamin Cummings, San Fransisco, CA.
Velkova Z, Stoytcheva M, Gochev V. 2012. Biosorption of Cu (II) onto chemically modified waste mycelium of Aspergillus awamori: equilibrium, kinetics and modeling studies. J Biosci Biotechnol 1(2): 163-169.
Xie X, Fu J, Wang H et al. 2010. Heavy metal resistance by two bacteria strains isolated from a copper mine tailing in China. Afr J Biotechnol 9(26): 4056-4066.
Zaidi A, Wani PA, Khan MS. 2012. Toxicity of Heavy Metals to Legumes and Bioremediation. Pondicherry, Springer
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