Short Communication: Genetic diversity of lemon (Citrus spp.) from Ternate Island (Indonesia) based on morphological and molecular characters




Abstract. Tolangara A, Corebima AD, Mas'ud A, Sundari. 2020. Short Communication: Genetic diversity of lemon (Citrus spp.) from Ternate Island (Indonesia) based on morphological and molecular characters. Biodiversitas 21: 1908-1913. One effort to build the image of Indonesian lemon can be by utilizing local genetic resources of Citrus spp. One of the growth centra lemon regions in North Maluku is Ternate Island. In Ternate island, cui (C. macrocarpa) is dominantly cultivated besides that there are Suanggi (C. histryx), nipis (C. aurantiifolia), and lemon (C sinensis). The genetic diversity of these local lemons in North Maluku, especially in Ternate, has not been scientifically reported. Therefore, this study aimed to analyze the genetic diversity of local lemons found on the island of Ternate based on morphological character and RAPD molecular analyses. A total of 12 accessions of local lemons in Ternate island to studied for morphological and molecular characters. Observation of morphological and morphometric characters of the lemons on 117 characters of vegetative and generative organs in lemons. Observation of molecular characters using three primers, i.e.: OPA1, OPA 2, and OPA 3 in the RAPD technique. The morphological character and molecular character using UPGMA analysis showed similar results. Ternate Island has moderate diversity of lemon species (75% based on morphological characters and 73% based on RAPD markers). Cluster analysis indicates that cui (C. macrocarpa) has the most distinctive autapomorphic character compared to the other local lemon varieties. Nipis (C.auratiifolia) and Suanggi (C. hystrix) shared 75% synapomorphic character similarity. This genetic diversity information provides a novel insight into the lemon commodity breeding programs which can be encouraged through the selection of superior local lemons based on morphological and molecular markers.


Adelina SO, Adelina E, Hasriyanty H. (2017). Morphological Identification and Anatomy of Local Oranges (Citrus SP) in Doda Village and Lempe Village, Lore Tengah District, Poso Regency. Jurnal Online AGROEKOTEKNOLOG, 5(1), 58–65[Indonesia].
Agriculture Department. 2007. Prospects and Direction of Development of Agricultural Commodities; Review of Land Resources Aspects. Agricultural Research and Development Agency. AGRO INNOVATION, 1-41[Indonesia].
Aydin O, Co?kunçelebi K, Gultepe M, Guzel ME. 2013. A contribution to taxonomy of Centaurea including Psephellus (Asteraceae) based on anatomical and molecular data. Turkish Journal of Botany, 37(3), 419-427.
Budiyati E, Nirmala FD. 2016. Utilization of Exploration Results of Nusantara Orange Germplasm. Jurnal AGROEKOTEKNOLOGI, 9(1), 58-66 [Indonesia].
Calinski T, Harabasz J. 1974. A dendrite method for cluster analysis. Communications in Statistics-theory and Methods. 3(1), 1-27.
Cottin, R. (2002). Citrus of the World, A Citrus Directory Version 2. SRA INRA-CIRAD, France.
Daryono BS, Subiastuti AS, Fatmadanni ASD. 2019. Phenotypic and genetic stability of new Indonesian melon cultivar (Cucumis melo L.‘Melonia’) based on ISSR markers. Biodiversitas Journal of Biological Diversity, 20(4), 1069-1075.
Davis P, Heywood HV. 1963. Principles of Angiosperm Taxonomy. Book., 556.
Ismail AY, Kusmana C, Sudiana E, Widodo P. 2019. Short Communication?: Population and stand structure of Cinnamomum sintoc in the Low Land Forest of Mount Ciremai National Park , West Java , Indonesia. Biodiversitas Journal of Biological Diversity, 20(4), 1042-1047.
John CS. 1965. Comparative Plant Anatomy?: A Quide to Taxonomic and Evolutionary Applications of Anatomical Data in Angiosperm. Holt, Renehart & Winston. New York, 146.
Machado, M.A., H.D.C. Filho, M.L.P.N. Targon, & Pompeu J. Jr. (1996). Genetic relationship of Mediterannean mandarins (Citrus deliciosa Tenore) using RAPD markers. Euphytica 92: 321-326.
Matakena S. 2017. Sweet Orange Commodity (Citrus Sinensis L) Agribusiness in Wadio Village, West Nabire District, Nabire Regency. Jurnal Fapertanak: Jurnal Pertanian Dan Peternakan, 2(1)[Indonesia].
Murtando H, Sahiri N, Madauna I. 2016. Identification of Morphological and Anatomical Characteristics of Local Citrus Plants (Citrus SP) in Karya Agung and Karya Abadi Village, Taopa District, Parigi Moutong District. E-j. AGROTEKBIS, 4(6), 642–649[Indonesia].
Karsinah, S., Setyobudi, L., & Aswidinnoor, H. (2002). Genetic diversity of orange germplasm based on analysis, RAPD marker. Journal of Agricultural Biotechnology, 7(1), 8-16
Kovach WL. 2007. Mutivariate Statistical Package (MVSP) Plus Version 3.22 User’s Manual. Publish by Kovach Computing Services. p. 137
Rezkianti V, Maemunah M, Lakani I. 2016. Morphological Identification and Anatomy of Local Oranges (Citrus SP.) In Hangira Village and Baleura Village, Lore Tengah District, Poso Regency. Jurnal Online AGROEKOTEKNOLOGI, 4(4), 412–418[Indonesia].
Solikin, (2012). Exploration of the local fruit of Madura, East Java. Proceedings of the National Seminar on Perhorti in collaboration with UPN "Veteran" East Java 2012. Building Synergy Stake Holders to Increase Competitiveness of Horticultural Products. Published April 2013 P 255-262Sturn A, Quackenbush J, Trajanoski Z. 2002. Genesis: cluster analysis of microarray data. Bioinformatics, 18(1), 207-208.
Sugiyarto, M. & Supriyanto A. (1992). Commencement of citrus plants. him. 92-106. Dalum A. Kasno, M. Dahlan, and Hasnam (Ed.) Proceedings of the East Java Regional Commisariat Plant Breeding Symposium. Matured Food Crops Research Institute.
Sundari Arumingtyas EL, Hakim L, Azrianingsi R, Wahyudi D. 2017. Genetic variability of local durian (durio zibethinus murr.) In ternate island based on rapd markers. Plant Cell Biotechnology and Molecular Biology, 18((1&2)), 68-75.
Sundari, Nurhasanah, Mas’ud A, Amin, M, Arumingtyas, E. L, & Azrianingsih, R. (2019). Update phylogenetic information of the local varieties of cloves (Syzygium aromaticum) from North Maluku, Indonesia based on ITS sequences data. Biodiversitas Journal of Biological Diversity, 20(6) e-ISSN: 2085-4722. 1510-1515 doi: 10.13057/biodiv/d200604 [Indonesia].
Sunaryono, H, (1982). Preservation of Fruits. Minutes of the Horticultural Workshop. Research and Development Center. Bogor pages: 64-68.
Stuessy TF. 1990. Plant Taxonomy; The Systematic Evaluation of Comparative Data. Columbia University Press. New York. 10pp.
Tjitrosoepomo G. 2009. Fundamentals of Plant Taxonomy (General Taxonomy). Penerbit Gadjah Mada University (UGM) Press, 582.
Tobing, Bayu, Siregar. 2013. Identification of Morphological Character in the Compilation of Description of Citrus (Citrus Nobilis) in Some Karo Districts. Jurnal Online AGROEKOTEKNOLOGI, 2(1), 72-85[Indonesia].
Williams DM. 2010. Plant Taxonomy: The Systematic Evaluation of Comparative Data. 2nd edition. Tod F. Stuessy, editor. Columbia University Press, 59(5), 608–610.