Variation of morphological characteristics, total phenolic, and total flavonoid in Adenostemma lavenia, A. madurense, and A. platyphyllum

##plugins.themes.bootstrap3.article.main##

NURLELA NURLELA
RIFAN NURFALAH
FIRA ANANDA
TAOPIK RIDWAN
AULIYA ILMIAWATI
WARAS NURCHOLIS
HIROSHI TAKEMORI
IRMANIDA BATUBARA

Abstract

Abstract. Nurlela, Nurfalah R, Ananda F, Ridwan T, Ilmiawati A, Nurcholis W, Takemori H, Batubara I. 2022. Variation of morphological characteristics, total phenolic, and total flavonoid in Adenostemma lavenia, A. madurense, and A. platyphyllum. Biodiversitas 23: 3999-4005. Adenostemma has long been used as a herbal medication. Despite some studies regarding the morphology and potency of Adenostemma carried out, the variation of morphological traits, total phenolic content (TPC), and total flavonoid content (TFC) on different species of Adenostemma has been poorly investigated. This study was designed to observe the morphological characteristics, TPC, and TFC of Adenostemma lavenia, A. madurense, and A. platyphyllum. Morphological characteristics were observed using qualitative and quantitative parameters. The colorimetric method determined TPC and TFC in plant leaf ethanol extracts. The plants were morphologically diverse, which significant variation among the three species in plant height, length and width of leaf, and internodes distance. Morphological traits such as leaf length and width, plant height, and internodes distance significantly correlate with phenolic and flavonoid content in Adenostemma. These may be used as important selection criteria in Adenostemma for obtaining high phenolic and flavonoid content. The study revealed that different species of these plants with varying morphological traits substantially impact the content of their chemical compounds. The highest TPC and TFC were found in A. lavenia, suggesting that it is potentially an alternative source of bioactive compounds as herbal medicine.

##plugins.themes.bootstrap3.article.details##

References
Ahmed SI, Hayat MQ, Tahir M, Mansoor Q, Ismail M, Keck K, Bates RB. 2016. Pharmacologically active flavonoids from the anticancer, antioxidant and antimicrobial extracts of Cassia angustifolia Vahl. BMC Complement Altern Med 16 (460): 1-9. DOI: 10.1186/s12906-016-1443-z.
Akhtar N, Ihsan-ul-Haq, Mirza B. 2018. Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species. Arab. J. Chem 11: 1223–1235. DOI: 10.1016/j.arabjc.2015.01.013.
Backer CA, Bakhuizen van den Brink Jr RC. 1965. Flora of Java, Volume II. N.V.P. Nordhoff, Groningen, Netherland.
Batubara I, Komariah K, Sandrawati A, Nurcholis W. 2020. Genotype selection for phytochemical content and pharmacological activities in ethanol extracts of fifteen types of Orthosiphon aristatus (Blume) Miq. leaves using chemometric analysis. Sci Rep 10 (20945): 1-11. DOI: 10.1038/s41598-020-77991-2.
Batubara I, Prastya, ME. 2020. Potential use of Indonesia medicinal plants for cosmetic and oral health: A review. Jurnal Kimia Valensi, 6(1): 118-132.
Batubara I, Prastya ME, Potensi Tanaman Rempah dan Obat Tradisional Indonesia Sebagai Sumber Bahan Pangan Fungsional. Komoditas Sumber Pangan untuk Meningkatkan Kualitas Kesehatan di Era Pandemi Covid -19; Prosiding Seminar Nasional Lahan Suboptimal ke-8, Palembang, 20 October 2020. [Indonesian].
Blair S, Madrigal B. 2005. Plantas antimaláricas de Tumaco: costa pacífica colombiana. Univ. de Antioquia, Medellín, Colombia.
Chen J-J, Deng J-S, Huang C-C, Li P-Y, Liang Y-C, Chou C-Y, Huang G-J. 2019. p-Coumaric-Acid-Containing Adenostemma lavenia Ameliorates Acute Lung Injury by Activating AMPK/Nrf2/HO-1 Signaling and Improving the Anti-oxidant Response. Am J Chin Med 47: 1483–1506. DOI: 10.1142/S0192415X19500769.
Christenhusz MJM, Byng JW. 2016. The number of known plants species in the world and its annual increase. Phytotaxa 261: 201. DOI: 10.11646/phytotaxa.261.3.1.
Chumroenphat T, Saensouk S, Saensouk P. 2021. Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand. Biodiversitas 22: 4036-4044. DOI: 10.13057/biodiv/d220952.
Elisha IL, Dzoyem J-P, McGaw LJ, Botha FS, Eloff JN. 2016. The anti-arthritic, anti-inflammatory, antioxidant activity and relationships with total phenolics and total flavonoids of nine South African plants used traditionally to treat arthritis. BMC Complement Altern Med 16 (307): 1-10. DOI: 10.1186/s12906-016-1301-z.
Ezez D, Tefera M. 2021. Effects of Solvents on Total Phenolic Content and Antioxidant Activity of Ginger Extracts. J. Chem. 2021: 1–5. DOI: 10.1155/2021/6635199.
Fauzan A, Praseptiangga D, Hartanto R, Pujiasmanto B. 2018. Characterization of the chemical composition of Adenostemma lavenia (L.) Kuntze and Adenostemma platyphyllum Cass. IOP Conf Ser: Earth Environ Sci 102 (012029): 1-8. DOI: 10.1088/1755-1315/102/1/012029.
Funk VA, Susanna A, Stuessy TF, Bayer RJ (eds). 2009. Systematics, evolution, and biogeography of the compositae. IAPT, International Association for Plant Taxonomy. Washington, DC, USA.
Iswantini D, Tuwalaid B, Trivadila. 2021. The Potency of Legetan warak (Adenostemma lavenia) and Kersen Leaf (Muntingia calabura) Extract as a Candidate for Chronic Obstructiv.e Pulmonary Disease (COPD) Herbal Medicine. Langsa, Aceh, Indonesia. 576: 447-452. DOI: 10.2991/assehr.k.210909.097.
Jeong KS, Heo TI, Lee KH, Choi K, Kim HJ. 2017. A New Record of Adenostemma madurense DC. (Asteraceae) in Korea. Korean J. Plant Res 30 (3): 331–334. DOI: 10.7732/KJPR.2017.30.3.331.
Kaur S, Mondal P. 2014. Study of Total Phenolic and Flavonoid Content, Antioxidant Activity and Antimicrobial Properties of Medicinal Plants. J. Microbol. Exp 1 (1): 1-6. DOI: 10.15406/jmen.2014.01.00005.
Khumaida N, Syukur M, Bintang M, Nurcholis W. 2019. Phenolic and flavonoid content in ethanol extract and agro-morphological diversity of Curcuma aeruginosa accessions growing in West Java, Indonesia. Biodiversitas 20: 656–663. DOI: 10.13057/biodiv/d200306.
Konieczynski P, Arceusz A, Wesolowski M. 2016. Essential Elements and Their Relations to Phenolic Compounds in Infusions of Medicinal Plants Acquired from Different European Regions. Biol Trace Elem Res 170: 466–475. DOI: 10.1007/s12011-015-0481-6.
Koyama H. 2002. Taxonomic Studies in the Compositae of Thailand 14. Tribe Eupatorieae, Bull. Natn. Sci 28 (2): 49- 60.
Koyama H, Sukhonthip B, Pimwadee P, DJ Nicholas H. 2016. Compositae, Asteraceae. In: Thawatchai S, Balslev H (eds). Flora of Thailand. Forest Herbarium, Royal Forest Department, Bangkok, Thailand.
Kumar S, Pandey AK. 2013. Chemistry and Biological Activities of Flavonoids: An Overview. Sci. World J. 2013: 1–16. DOI: 10.1155/2013/162750.
Laldingliani TBC, Thangjam NM, Zomuanawma R, Laldingngheti B, Pal A, Kumar A. 2022. Ethnomedicinal study of medicinal plants used by Mizo tribes in Champhai district of Mizoram, India. J. Ethnobiology Ethnomedicine 18 (22): 1-29. DOI: 10.1186/s13002-022-00520-0.
Lattanzio V. 2013. Phenolic Compounds: Introduction. In: Ramawat KG, Mérillon J-M (eds) Natural Products. Springer Berlin Heidelberg, Berlin.
Maeda M, Suzuki M, Fuchino H, Tanaka N, Kobayashi T, Isogai R, Batubara I, Iswantini D, Matsuno M, Kawahara N, Koketsu M, Hamamoto A, Takemori H. 2022. Diversity of Adenostemma lavenia, multi-potential herbs, and its kaurenoic acid composition between Japan and Taiwan. J Nat Med 76: 132–143. DOI: 10.1007/s11418-021-01565-3.
Moncayo S, Cornejo X, Castillo J, Valdez V. 2021. Preliminary phytochemical screening for antioxidant activity and content of phenols and flavonoids of 18 species of plants native to western Ecuador. Trends Phytochem. Res 5 (2): 92-104. DOI: 10.30495/TPR.2021.1922658.1196.
Orchard AE. 2011. A review of Australian Adenostemma J.R.Forst. & G.Forst. (Asteraceae: Eupatorieae). Telopea 13 (1–2): 341–348.
Parveen A, Parveen B, Parveen R, Ahmad S. 2015. Challenges and guidelines for clinical trial of herbal drugs. J Pharm Bioall Sci 7 (4): 329-333. DOI: 10.4103/0975-7406.168035.
Praptiwi P, Sulistiarini D, Qodrie ENP, Sahroni D. 2021. Antibacterial activity, antioxidant potential, total phenolic and flavonoids of three plant species of Rubiaceae from Banggai Island, Indonesia. Biodiversitas 22 (5): 2773-2778. DOI: 10.13057/biodiv/d220540.
Prasad AGD, Shyma TB, Raghavendra MP. 2013. Plants used by the tribes for the treatment of digestive system disorders in Wayanad district, Kerala. J. Appl. Pharm. Sci 3 (08): 171-175. DOI: 10.7324/JAPS.2013.3830.
Ren J, Ji X, Wang C, Hu J, Nervo G, Li J. 2020. Variation and Genetic Parameters of Leaf Morphological Traits of Eight Families from Populus simonii × P. nigra. Forests 11 (1319): 1-17. DOI: 10.3390/f11121319.
Teixeira TS, Vale RC, Almeida RR, Ferreira TPS, Guimaraes LGL. 2017. Antioxidant Potential and its Correlation with the Contents of Phenolic Compounds and Flavonoids of Methanolic Extracts from Different Medicinal Plants. Rev Virtual Quim 9 (4):1546–1559. DOI: 10.21577/1984-6835.20170090.
Sabir SM, Athayde ML, Boligon AA, Rocha JBT. 2017. Antioxidant activities and phenolic profile of Baccharis trimera, a commonly used medicinal plant from Brazil. S. Afr. J. Bot 113: 318–323. DOI: 10.1016/j.sajb.2017.09.010.
Suryani S, Al Anshory AC, Marlin, Artika IM, Ambarsari L, Nurcholis W. 2022. Variability total phenolic content and antioxidant activity of Curcuma zanthorrhiza and C. aeruginosa cultivated in three different locations in West Java, Indonesia. Biodiversitas 23 (4): 1998-2003. DOI: 10.13057/biodiv/d230434.
Svobodova B, Barros L, Calhelha RC, Heleno S, Alves MJ, Walcott S, Bittova M, Kuban V, Ferreira ICFR. 2017. Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago. Ind. Crops Prod 95: 365–373. DOI: 10.1016/j.indcrop.2016.10.046.
Takaidza S, Mtunzi F, Pillay M. 2018. Analysis of the phytochemical contents and antioxidant activities of crude extracts from Tulbaghia species. J. Tradit. Chin. Med 38: 272–279. DOI: 10.1016/j.jtcm.2018.04.005.
Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. 2018. Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines 5 (93): 1-16. DOI: 10.3390/medicines5030093.
Valentin-Silva A, Godinho MAS, Vieira MF. 2016. Life history of Adenostemma brasilianum (Pers.) Cass. (Eupatorieae, Asteraceae): A psychophilous herbaceous species of the Brazilian Atlantic Forest understory. J. Torrey Bot. Soc 143 (1): 87–92. DOI: 10.3159/TORREY-D-14-00088.1.
Wu Z, Raven PH, Hong D, Missouri Botanical Garden (eds). 1994. Flora of China. Missouri: St. Louis.
Z?otek U, Szymanowska U, Pecio ?, Kozachok S, Jakubczyk. 2019. Antioxidative and Potentially Anti-inflammatory Activity of Phenolics from Lovage Leaves Levisticum officinale Koch Elicited with Jasmonic Acid and Yeast Extract. Molecules 24 (1441): 1-12. DOI: 10.3390/molecules24071441.