Assessment of in vitro activities and chemical profiling of Senecio hoggariensis growing in Algerian Sahara




Abstract. Arab Y, Sahin B, Ceylan O, Zellagui A, Olmez OT, Kucukaydin S, Tamfu AN, Ozturk M, Gherraf N. 2022. Assessment of in vitro activities and chemical profiling of Senecio hoggariensis growing in Algerian SaharaBiodiversitas 23: 3498-3506The in vitro antioxidant, anticholinesterase, tyrosinase inhibitory, antibiofilm, and anti-quorum sensing activities of the ethyl acetate extract of Senecio hoggariensis,growing in Algerian Sahara, were studied along with its chemical constituents using HPLC-DAD. The chromatographic analysisunveiled seven phenolic compounds, including p-coumaric acid as a major component. Additionally, the extract showed moderate DPPH radical scavenging activity, compared to known standards. At 200 ?g/mL, the extract disclosed equitable acetylcholinesterase (AChE), butyryl-cholinesterase (BChE) and tyrosinase inhibition rates with respective values of 37.01±1.48%, 18.87±4.18% and 45.99±1.81%. Likewise, the extract exhibited a good antibiofilm activity against Candida albicans ATCC 10239 biofilm production with an inhibition ratio of 59.56±0.40%, at 50µg/mL. The anti-quorum sensing by QS-regulated violacein pigment production inhibition test was determined using Chromobacterium violaceum CV026 and CV12472. The swarming motility inhibition assay was determined using Pseudomonas aeruginosa PA01. It is evident from the findings that Senecio hoggariensis could be considered potential antioxidant, anti-QS, and antibiofilm compounds. However, the origin of anti-biofilm and anti-quorum sensing activities of the ethyl acetate extract could be revealed by further studies on the mechanism of action of active compounds that can be isolated via activity-guided fractionation.


ALBAYRAK S, AKSOY A, YURTSEVEN L, YA?AR A. 2016. A Comparative Study on Antioxidant and Antibacterial Activities of Four Senecio Species From Turkey. International Journal of Secondary Metabolite. 2(2):26–36.
Benso B, Lespay-Rebolledo C, Flores L, Zárraga M, Brauchi S. 2018. Chalcones Derivatives as Potent Inhibitors of TRPV1 Activity. Biophysical Journal. 114(3):483a.
Bousetla A, Keskinkaya HB, Bensouici C, Lefahal M, Atalar MN, Akkal S. 2021. LC-ESI/MS-phytochemical profiling with antioxidant and antiacetylcholinesterase activities of Algerian Senecio angulatus L.f. extracts. Natural Product Research.:1–7.
Conforti F, Loizzo MR, Statti GA, Houghton PJ, Menichini F. 2006. Biological properties of different extracts of two Senecio species. International Journal of Food Sciences and Nutrition. 57(1-2):1–8.
Conforti F, Marrelli M, Statti G, Menichini F. 2006. Antioxidant and cytotoxic activities of methanolic extract and fractions from Senecio gibbosussubsp.gibbosus(GUSS) DC. Natural Product Research. 20(9):805–812.
Durak MZ, Uçak G. 2015. Solvent optimization and characterization of fatty acid profile and antimicrobial and antioxidant activities of Turkish Pistacia terebinthus L. extracts. Turkish Journal of Agriculture and Forestry. 39:10–19.
Gupta M, Karmakar N, Sasmal S, Chowdhury S, Biswas S. 2016. Free radical scavenging activity of aqueous and alcoholic extracts of Glycyrrhiza glabra Linn. measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (?TEAC), DPPH assay and peroxyl radical antioxidant assay. International Journal of Pharmacology and Toxicology. 4(2):235.
Husain FM, Ahmad I. 2013. Quorum sensing inhibitors from natural products as potential novel antiinfective agents. Drugs of the Future. 38(10):691.
Koh KH, Tham F-Y. 2011. Screening of traditional Chinese medicinal plants for quorum-sensing inhibitors activity. Journal of Microbiology, Immunology and Infection. 44(2):144–148.
Koz?owska M, Gruczy?ska E, ?cibisz I, Rudzi?ska M. 2016. Fatty acids and sterols composition, and antioxidant activity of oils extracted from plant seeds. Food Chemistry. 213:450–456.
Lahlou F. 2014. Antioxidant Activity Phenolics Flavonoids and Proanthocyanidins Content of Senecio anteuphorbium. International Journal of Biochemistry Research & Review. 4(6):550–558.
Lebrun JP. 1981. Les bases floristiques des grandes divisions chorologiques de l’Afrique seche. [place unknown]: Institut D’elevage Et De Medecine Veterinaire Des Pays Tropicaux.
Lou Z, Wang H, Rao S, Sun J, Ma C, Li J. 2012. p-Coumaric acid kills bacteria through dual damage mechanisms. Food Control. 25(2):550–554.
Maryam S, Pratama R, Effendi N, Naid T. 2016. Analisis Aktivitas Antioksidan Ekstrak Etanolik Daun Yodium (Jatropha multifida L.) DENGAN Metode Cupric Ion Reducing Antioxidant Capacity (CUPRAC). Jurnal Fitofarmaka Indonesia. 2(1).
Milad R. 2014. Genus Kalanchoe (Crassulaceae): A Review of Its Ethnomedicinal, Botanical, Chemical and Pharmacological Properties. European Journal of Medicinal Plants. 4(1):86–104.
Min Chang T. 2012. Tyrosinase and Tyrosinase Inhibitors. Journal of Biocatalysis & Biotransformation. 01(02).
Othman AFM, Rukayadi Y, Radu S. 2019. Inhibition of Pseudomonas aeruginosa Quorum Sensing by Curcuma xanthorrhiza Roxb. Extract. Journal of Pure and Applied Microbiology. 13(3):1335–1347.
Öztürk M, Tel G, Öztürk FA, Duru ME. The cooking effect on two edible mushrooms in Anatolia: fatty acid composition, total bioactive compounds, antioxidant and anticholinesterase activities. Records of Natural Products. 2014;8(2):189.
?ahin S. 2013. Evaluation of Antioxidant Properties and Phenolic Composition of Fruit Tea Infusions. Antioxidants. 2(4):206–215.
Saurav K, Costantino V, Venturi V, Steindler L. 2017. Quorum Sensing Inhibitors from the Sea Discovered Using Bacterial N-acyl-homoserine Lactone-Based Biosensors. Marine Drugs. 15(3):53.
Suleria HAR, Barrow CJ, Dunshea FR. 2020. Screening and Characterization of Phenolic Compounds and Their Antioxidant Capacity in Different Fruit Peels. Foods. 9(9):1206.
Sybiya Vasantha Packiavathy IA, Agilandeswari P, Musthafa KS, Karutha Pandian S, Veera Ravi A. 2012. Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against Gram negative bacterial pathogens. Food Research International. 45(1):85–92.
Tamfu AN, Tagatsing Fotsing M, Talla E, Ozturk M, Mbafor JT, Duru ME, Shaheen F. 2019. Chemical composition and evaluation of anticholinesterase activity of essential oil from Cameroonian propolis. Issues in Biological Sciences and Pharmaceutical Research. 7(3).
Tidjani S, Okusa PN, Zellagui A, Banuls LMY, Stévigny C, Duez P, Rhouati S. 2013. Analysis of Pyrrolizidine Alkaloids and Evaluation of Some Biological Activities of Algerian Senecio Delphinifolius (Asteraceae). Natural Product Communications. 8(4):1934578X1300800.
Tokul-Ölmez Ö, Sahin B, Çak?r C, Ozturk M. 2020. Rapid and easy method for simultaneous measurement of widespread 27 compounds in natural products and foods. Journal of Chemical Metrology. 14(1):1–11.
Wang X, Stone HA, Golestanian R. 2017. Shape of the growing front of biofilms. New Journal of Physics. 19(12):125007.
Yue Y, Shen P, Xu Y, Park Y. 2018. p-Coumaric acid improves oxidative and osmosis stress responses in Caenorhabditis elegans. Journal of the Science of Food and Agriculture. 99(3):1190–1197.