Naphthalene degradation by Pseudomonas sp. strain LBKURCC149 with the addition of glucose as co-substrate

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DOI https://doi.org/10.13057/biodiv/d231115
Issue
Vol. 23 No. 11 (2022)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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RIRYN NOVIANTY ♥
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. H.R. Subrantas, Km. 12,5, Simpang Baru, Pekanbaru 28292, Riau, Indonesia
BHEKTI ANTIKA
Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. H.R. Subrantas, Km. 12,5, Simpang Baru, Pekanbaru 28292, Riau, Indonesia
SARYONO
Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. H.R. Subrantas, Km. 12,5, Simpang Baru, Pekanbaru 28292, Riau, Indonesia
AMIR AWALUDDIN
Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. H.R. Subrantas, Km. 12,5, Simpang Baru, Pekanbaru 28292, Riau, Indonesia
NOVA WAHYU PRATIWI
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Riau. Kampus Bina Widya, Jl. H.R. Subrantas, Km. 12,5, Simpang Baru, Pekanbaru 28292, Riau, Indonesia
ERWINA JULIANTARI
Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor. Jl. Agatis, Kampus IPB Dramaga, Bogor 16680, West Java, Indonesia

Abstract

Abstract. Novianty R, Antika B, Saryono, Awaluddin A, Pratiwi NW, Juliantari E. 2022. Naphthalene degradation by Pseudomonas sp. strain LBKURCC149 with the addition of glucose as co-substrate. Biodiversitas 23: 5654-5661. Naphthalene is one of the pollutants that cause carcinogens. The biodegradation of carcinogenic naphthalene toxic compounds needs to be done to control pollution. Pseudomonas is one of several bacterial species that can degrading naphthalene. This study aimed to test the effectiveness of Pseudomonas sp. isolated from the land area of ??the Joint Operating Body (JOB) of PT. Siak Bumi Pusako-Pertamina Hulu in degrading naphthalene with glucose as a co-substrate. The potency of three isolates of Pseudomonas sp. LBKURCC148, LBKURCC149, and LBKURCC150 was tested in Minimal Media (MM) containing 0.2 mM naphthalene and incubated for seven days. The Optical Density (OD), pH, decreased concentration of naphthalene and degradation ability parameters were analyzed using UV-Vis spectrophotometer. The results showed that Pseudomonas sp. LBKURCC149 was able to degrade naphthalene by 13.95%. Naphthalene analysis using HPLC resulted in a decrease in the peak area of ??the sample as compared to the control. Based on these findings Pseudomonas sp. LBKURCC149 could be recommended to use to degrade naphthalene pollution.

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References
Abarian M, Hassanshahian M, Badoei-Dalfard A. 2018. Isolation, screening, and characterization of naphthalene-degrading bacteria from Zarand Mine, Iran. Polycyclic Aromatic Compounds. 38(5): 410-419
Aina OE, Agbaji EB, Nwokem NC. 2018. Biodegradation of naphthalene using Pseudomonas putida and Bacillus substilis immobilized on snail shell. Journal of Application Environmental Management. 22(7):1065-1070.
Al-Hawash AB, Dragh MA, Li S, Alhujaily A, Abbood HA, Zhang X, Ma F. 2018. Principles of microbial degradation of petroleum hydrocarbons in the environment. Egypt. J. Aquat. Res. 44 (2), 71–76.
Atlas RM. 1981. Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiological Reviews. 45(1): 180-20.
Azubuike CC, Chikere CB, Okpokwasili GC. 2016. Bioremediation techinques-classification based on site of application: principle, advantegaes, limitation and prospects. 32(180): 1-18.
Bisht S, Pandey P, Bhargava B, Sharma S, Kumar V, Sharma KD, Bioremediation of polyaromatic hydrocarbons (PAHs) using rhizosphere technology. Braz J Microbiol. 46(1): 1-12.
Bren A, Park JO, Towbin BD, Dekel E, Rabinowitz JD, Alon U .2016. Glucose becomes one of the worst carbon sources for E.coli on poor nitrogen sources due to suboptimal levels of cAMP. Sci Rep. 6:24834.
Bonfa MR, Grossman MJ, Mellado E, Durrant LR. 2011. Biodegradation of aromatic hydrocarbons by Haloarchaea and their use for the reduction of the chemical oxygen demand of hypersaline petroleum produced water. Chemosphere. 84: 1671–1676.
Bourguinon N, Isaac P, Alvarez H, Amoroso MJ, Ferrero MA. 2014. Enhanced polyaromatic hydrocarbon degradation by adapted cultures of actinomycete strains. Journal of Basic Microbiology. 54(1):1-7.
Chang WT, Fang MD, Lee CL, Brimblecombe P. 2014. Measuring bioavailable PAHs in estuarine water using semipermeable membrane devices with performance reference compounds. Mar. Pollut. Bull. 89: 376–383.
Farini N, Thontowi A, Yetti E, Yopi S. 2017. Pertumbuhan bakteri laut Shewanella indica lbf-1-0076 dalam naftalena dan deteksi gen naftalena. J. Biopropal. Ind. 8:19–31.
Fitrida A, Apriyana K, Astuti R, Novianty R. 2020a. Biodegradation of environmental pollutant naphthalene toxic compounds using indigenous fungi isolates in Riau Province. J Ipteks Terapan 13:165-174. [Indonesian]
Fitrida A, Novianty R, Saryono, Awaluddin, A, Pratiwi NW. 2020b. Optimization of the growth of isolates of indigenous Penicillium sp. LBKURCC153 naphthalene degrading using glucose as cosubstrate in minimal medium. J Inov Pend IPA 1: 20-25. [Indonesian]
Ghosal D, Ghosh S, Dutta TK, Ahn Y. 2016. Current state of knowledge in microbial degradation of polycyclic aromatic hydrocarbons (PAHs): A Review. Front Microbiol. 15(7):1837.
Hussein IAS, Mansour MSM. 2016. A review on polycyclic aromatic hydrocarbons: Source, environmental impact, effect on human health and remediation. Egyptian Journal of Petroleum. 25(1): 107-123
Hatef ZH, Khudeir SH. 2015. Optimal conditions for naphthalene biodegradation by local bacterial isolates. International Journalof Science dan Research. 6(12): 1790-1795.
Janbandhu A, Fulekar MH. 2011. Biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment. Journal of Hazardous Materials. 187(3): 333-340.
Juliani A, Lathifah AN. Nurdin WA. 2016. Pengaruh penambahan kosubstrat pada biodegradasi crude oil. Jurnal Sains dan Teknologi Lingkungan. 8(2): 112-120.
Lee SD, Lee M.W, Woo SH, Park JM. 2005. Effects os salycilate and glucose on biodegradation of phenanthrene by Burkholderia cepacia PM07. J. Microbiol Biotech. 15: 859-865.
Malatova K. 2015, Isolation and characterization of hydrocarbon degrading bacteria from environmental habitats in western New York State. Thesis. Rochester: Rochester Institute of Technology.
Mesbaiah FZ, Mansour F, Eddouaouda K, Badis A. 2014. Surfactant effect on biodegradation of polycyclic aromatic hydrcarbons. Desalination and Water Treatment. 1(1): 1-6.
Nezhad AK, Ghasemi M, Khosravan A, Shakibaie M. 2015. Cadmium Bioremediation by Metal-Resistant Mutated Bacteria Isolated from Active Sludge of Industrial Effluent. J. Environ. Health Sci. Eng. 7: 279-285.
Nielsen CK, Kjems J, Mygind T, Snabe T, Meyer RL. 2016. Effects of tween 80 on growth and biofilm formation in laboratory media. Front Microbiol. 2016 Nov 22;7:1878.
Novianty R, Awaluddin A, Pratiwi NW. 2020a. Indigenous bacteria degrading petroleum hydrocarbons in Siak Regency, Riau Province. J Teknik Kimia USU 1: 34-40. [Indonesian]
Novianty R, Antika B, Saryono S, Awaluddin A, Pratiwi NW. 2020b. The potential of three isolates of indigenous bacteria from Siak Regency, Riau Province in degrading naphthalene. J Kimia (Journal of Chemistry) 14: 94-100. [Indonesian]
Novianty R, Saryono, Awaluddin A, Pratiei NW, Hidayah A, Juliantari E. 2021. The diversity of fungi consortium isolated from polluted soil for degrading petroleum hydrocarbon. Biodiversitas. 22(11): 5077-5084.
Pawar AN, Ugele SS, More MG, Kokani NF, Khandelwan SR. 2013.. Biological degradation of naphthalene: A New Era. J. Bioremed. Biodegradation. 4: 4-5.
Rabani MS, Sharma R, Singh R, Gupta MK. 2020. Characterization and Identification of Naphthalene Degrading Bacteria Isolated from Petroleum Contaminated Sites and Their Possible Use in Bioremediation. Polycyclic Aromatic Compounds.
Raissa KR, Augustin M, Benoit NM. 2012. Naphthalene biodegradation by microbial consortia isolated from soils in Ngaoundere (Cameroon). Int. J. Environ. Sci. 3: 1-10.
Sharah A, Karnila R, Desmelati. 2015. Pembuatan kurva pertumbuhan bakteri asam laktat yang di isolasi dari ikan Peda Kembung (Rastrellinger sp.). JOM. 2(1): 1-8.
Sari EM, Novianty R, Awaluddin A, Saryono. 2019. Effectiveness of Crude Oil Degrading Fungi Isolated From Petroleum Hydrocarbon Contaminated Soil in Siak, Riau. J. Acta Biochimica Indonesiana. 2: 15-22.
Siota LF, Santos F, Miguel T, Villa T. 2008. Biodegradation of naphthalene by Pseudomonas stutzeri in marine environments: testing cells entrapmentin calcium alginate for use in water detoxification. Bioremediation Journal. 12(4): 185-192.
Sudrajat Dadan., Nana, Mulyana T. R. D. 2015. Isolasi dan aplikasi mikroba indigen pendegradasi hidrokarbon dari tanah tercemar minyak bumi. Prosiding Pertemuan dan Presentasi Ilmiah – Penelitian Dasar Ilmu Pengetahuan dan Teknologi Nuklir 2015. Pusat Sains dan Teknologi Akselerator BATAN, Yogyakarta.
Sumar, H. 2006. Kimia Pemisahan Metode Kromatografi dan Elektroforesis Modern. Bandung: Remaja Rosdakarya Offset.
Su WT, Wu BS, Chen WJ. Characterization and biodegradation of motor oil by indigenous Pseudomonas aeruginosa and optimizing medium constituents. J Taiwan Inst Chem Eng. 2011;42:689–695.
Tirkey SR, Ram S, Mishra S. 2021. Naphthalene degradation studies using Pseudomonas sp. strain SA3 from Alang-Sosiya ship breaking yard, Gujarat. Heliyon 7(2021): e06334
Travkin, V.M.; Solyanikova, I.P. 2021. Salicylate or phthalate: the main intermediates in the bacterial degradation of naphthalene. Processes. 9(1862): 1-18.
Vanishree M, Thatheyus AJ, Ramya D. 2014. Biodegradation of petrol using fungus Peniciliium sp. Sci Int. 2(1): 26-31..
Wijayaratih Y. 2001. Perombakan senyawa hidrokarbon aromatis polisiklik (naftalen) pada kadar tinggi oleh Pseudomonas NY-1. J. Manusia dan Lingkungan. 8: 130-141.
Xu X, Liu W, Tian S, Wang W, Qi Q, Jiang P, Gao X, Li F, Li H, Yu H. 2018. Petroleum hydrocarbon-degrading bacteria for the remediation of oil pollution under aerobic conditions: a perspective analysis. Front Microbiol 9: 1-11.
Yudono B, Estuningsih SP, Said M, Napoleon A. Eksplorasi Bakteria Indigen Pendegradasi Limbah Minyak Bumi di Wilayah PT Pertamina UBEP Limau Muara Enim. Prosiding Semirata FMIPA Universitas Lampung. Lampung, 21 November 2013.