Physical properties, nutritional composition, and antioxidant profiles of five varieties of pumpkin (Cucurbita moschata) seeds

Main Article Content

NURRAHMAN
AGUS SUYANTO
RAHAYU ASTUTI
YUNIKE KUSUMA DEWI
NUR AMALIA

Abstract

Abstract. Nurrahman, Suyanto A, Astuti R, Dewi YK, Amalia N. 2026. Physical properties, nutritional composition, and antioxidant profiles of five varieties of pumpkin (Cucurbita moschata) seeds. Asian J Agric 10 (1): g100132. https://doi.org/10.13057/asianjagric/g100132. Pumpkin (Cucurbita moschata) seeds, in terms of their chemical and nutritional components, have potential as a functional food. The bioactive components contained in pumpkin seeds include carotenoids, tocopherols, antioxidants, and sterols. Pumpkin seed is also rich in bioactive compounds, such as phytosterols, which have antioxidant activity and potential for pharmacological research. The aim of this research is to determine the physical properties, nutritional composition, and antioxidant profiles of five varieties of pumpkin seeds. The research was conducted on five varieties of pumpkin; the pumpkins were cut in half and the seeds were removed. Then, the weight and volume of the seeds were measured. After that, the tested composition covered levels of proximate, fiber, fatty acids, amino acids, β-carotene, total phenols, antioxidant activity, and vitamins C and E. The data obtained were analyzed using the ANOVA method, and the LSD method was used to see the differences between each variety. Research results showed that the five varieties of pumpkin seeds had physical, compositional, nutritional, and antioxidant characteristics, as well as amino acids, that vary. The most common amino acids from the various varieties were glutamic acid, arginine, and aspartic acid. The seeds of Kabocha pumpkin had a composition of proximate, nutrients, antioxidants, and amino acids that are higher compared to other pumpkin seeds varieties. Meanwhile, the highest fatty acids were in the Ketan variety. Different varieties of pumpkin seeds had different physical and chemical characteristics.

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NURRAHMAN, N., SUYANTO, A., ASTUTI, R., DEWI, Y. K., & AMALIA, N. (2026). Physical properties, nutritional composition, and antioxidant profiles of five varieties of pumpkin (Cucurbita moschata) seeds. Asian Journal of Agriculture, 10(1). https://doi.org/10.13057/asianjagric/g100132

References

Abdullah M, Jamil RT, Attia FN. 2023. Vitamin C (Ascorbic Acid). StatPearls Publishing, Treasure Island (FL).

Altaf A, Sharma H, Dar AH, Dash KK, Pandey VK, Manzoor M. 2025. Phytochemical potential and biological activity of pumpkin: A review. Food Saf Health 3 (2): 188-201. https://doi.org/10.1002/fsh3.70001.

Amin MZ, Islam T, Uddin MR, Uddin MJ, Rahman MM, Satter MA. 2019. Comparative study on nutrient contents in the different parts of indigenous and hybrid varieties of pumpkin (Cucurbita maxima L.). Heliyon 5 (9): e02462. https://doi.org/10.1016/j.heliyon.2019.e02462.

Anggreni N, Mulyaningsih S, Bachri MS. 2024. Aktivitas antioksidan serta penetapan kadar β-karoten, flavonoid dan fenol total ekstrak etanol labu kuning (Cucurbita moschata (Duch.) Poir.). Jurnal Insan Farmasi Indonesia 7 (3): 345-355. https://doi.org/10.36387/jifi.v7i3.2221. [Indonesian]

Arifin AG, Ainurrohmah N, Ardiarini NR, Sugiharto AN. 2024. Efek xenia dan metaxenia pada persilangan labu kuning (Cucurbita maxima Duch.) terhadap buah dan biji. PLANTROPICA: J Agric Sci 9 (1): 33-43. http://dx.doi.org/10.21776/ub.jpt.2024.009.1.4. [Indonesian]

Association of Officiating Analytical Chemists (AOAC). 2005. Official Method of Analysis. 18th Edition. AOAC, Washington DC.

Bardaa S, Halima NB, Aloui F, Mansour RB, Jabeur H, Bouaziz M, Sahnoun Z. 2016. Oil from pumpkin (Cucurbita pepo L.) seeds: Evaluation of its functional properties on wound healing in rats. Lipids Health Dis 15: 73-84. https://doi.org/10.1186/s12944-016-0237-0.

Batool M, Ranjha MMAN, Roobab U, Manzoor MF, Farooq U, Nadeem HR, Nadeem M, Kanwal R, AbdElgawad H, Al Jaouni SK, Selim S, Ibrahim SA. 2022. Nutritional value, phytochemical potential, and therapeutic benefits of pumpkin (Cucurbita sp.). Plants 11 (11): 1394. https://doi.org/10.3390/plants11111394.

Darmakusuma D, Boeky GC, Ola ARB, Suwari, Dje YCB, Bekak Y, Neto PD, Mutis A. 2025. Antioxidant activity and total phenol content in steamed pumpkin (Cucurbita moschata) flour with skin colour variations. Int J Sci Res Mod Technol 4 (1): 32-36. https://doi.org/10.5281/zenodo.14752649.

Devi NM, Prasad R, Sagarika N. 2018. A review on health benefits and nutritional composition of pumpkin seeds. Intl J Chem Stud 6 (3): 1154-1157.

Dhurhania CE, Novianto A. 2018. Uji kandungan fenolik total dan pengaruhnya terhadap aktivitas antioksidan dari berbagai bentuk sediaan sarang semut (Myrmecodia pendens). Jurnal Farmasi dan Ilmu Kefarmasian Indonesia 5 (2): 62-68. https://doi.org/10.20473/jfiki.v5i22018.62-68. [Indonesian]

Dotto JM, Chacha JS. 2020. The potential of pumpkin seeds as a functional food ingredient: A review. Sci Afr 10: e00575. https://doi.org/10.1016/j.sciaf.2020.e00575.

Dowidar MF, Ahmed AI, Mohamed HR. 2020. The critical nutraceutical role of pumpkin seeds in human and animal health: An updated review. Zagazig Vet J 48 (2): 199-212. https://doi.org/10.21608/zvjz.2020.22530.1097.

Dyshlyuk L, Babich O, Prosekov A, Ivanova S, Pavskya V, Yang Y. 2017. In vivo study of medical and biological properties of functional bakery products with the addition of pumpkin flour. Bioact Carbohydr Diet Fibre 12: 20-24. https://doi.org/10.1016/j.bcdf.2017.09.001

Gade SR, Meghwal M, Prabhakar PK, Giuffrè AM. 2022. A comparative study on the nutritional, antioxidant, thermal, morphological and diffraction properties of selected cucurbit seeds. Agronomy 12 (10): 2242. https://doi.org/10.3390/agronomy12102242.

Grajzer M, Kozłowska W, Zalewski I, Matkowski A, Wiland-Szymańska J, Rękoś M, Prescha A. 2025. Nutraceutical prospects of pumpkin seeds: A study on the lipid fraction composition and oxidative stability across eleven varieties. Foods 14 (3): 354. https://doi.org/10.3390/foods14030354.

Gulcin İ. 2025. Antioxidants: A comprehensive review. Arch Toxicol 99: 1893-1997. https://doi.org/10.1007/s00204-025-03997-2.

Gumolung D. 2018. Analisis kandungan total fenolik pada jonjot buah labu kuning (Cucurbita moschata). Fullerene J Chem 3 (1): 1-4. https://doi.org/10.37033/fjc.v3i1.25. [Indonesian]

Gutierrez RMP. 2016. Review of Cucurbita pepo (pumpkin) its phytochemistry and pharmacology. Med Chem 6 (1): 12-21. https://doi.org/10.4172/2161-0444.1000316.

Habib M, Singh S, Sagar NA, Ahmad S, Qureshi I, Jan S, Jan K, Bashir K. 2025. Physicochemical and functional characterization of pumpkin seed protein isolate. Sustain Food Technol 3 (2): 445-455. https://doi.org/10.1039/d4fb00268g.

Hussain A, Kausar T, Jamil MA, Noreen S, Iftikhar K, Rafique A, Iqbal MA, Majeed MA, Quddoos MY, Aslam J, Ali A. 2022. In vitro role of pumpkin parts as pharma-foods: Antihyperglycemic and antihyperlipidemic activities of pumpkin peel, flesh, and seed powders in alloxan-induced diabetic rats. Intl J Food Sci 2022: 1-10. https://doi.org/10.1155/2022/4804408.

Kar S, Dutta S, Yasmin R. 2023. A comparative study on phytochemicals and antioxidant activity of different parts of pumpkin (Cucurbita maxima). Food Chem Adv 3: 100505. https://doi.org/10.1016/j.focha.2023.100505.

Kinsman ME, Serviss MT, Meru G, Chase CA, Sargent SA, Simonne A, MacIntosh AJ. 2024. A greener approach to assess bioactive compounds in tropical pumpkin (Cucurbita moschata) using colorimetry. Front Sustain Food Syst 8: 1480964. https://doi.org/10.3389/fsufs.2024.1480964.

Koike K, Li W, Liu L, Hata E, Nikaido T. 2005. New phenolic glycosides from the seeds of Cucurbita moschata. Chem Pharm Bull 53 (2): 225-228. https://doi.org/10.1248/cpb.53.225.

Lestari BE, Meiyanto E. 2018. A review: The emerging nutraceutical potential of pumpkin seeds. Indones J Cancer Chemoprevent 9 (2): 92-101. http://dx.doi.org/10.14499/indonesianjcanchemoprev9iss2pp92-101.

Li FS, Xu J, Dou DQ, Chi XF, Kang TG, Kuang HX. 2009. Structures of new phenolic glycosides from the seeds of Cucurbita moschata. Nat Prod Commun 4 (4): 511-512. https://doi.org/10.1177/1934578X0900400413.

Lopez MJ, Mohiuddin SS. 2024. Biochemistry, Essential Amino Acids. StatPearls Publishing, Treasure Island (FL).

Manshi, Chaturvedi N. 2023. Potential pharmacological activities of pumpkin seeds as a functional food: A comprehensive review. J Ayurvedic Herb Med 9 (2): 101-107. https://doi.org/10.31254/jahm.2023.9209.

Medjakovic S, Hobiger S, Ardjomand-Woelkart K, Bucar F, Jungbauer A. 2016. Pumpkin seed extract: Cell growth inhibition of hyperplastic and cancer cells, independent of steroid hormone receptors. Fitoterapia 110: 150-156. https://doi.org/10.1016/j.fitote.2016.03.010.

Mondaca RL, Marin J, Rivas J, Sanhueza L, Soto Y, Vera N, Díaz LP. 2019. Pumpkin seeds (Cucurbita maxima): A review of functional attributes and by-products. Rev Chil Nutr 46 (6): 783-791. https://doi.org/10.4067/S0717-75182019000600783.

Montesano D, Blasi F, Simonetti MS, Santini A, Cossignani L. 2018. Chemical and nutritional characterization of seed oil from Cucurbita maxima L. (var. Berrettina) pumpkin. Foods 7 (3): 30. https://doi.org/10.3390/foods7030030.

Nurrahman, Astuti R. 2022. Analisis komposisi zat gizi dan antioksidan beberapa varietas labu kuning (Cucurbita moschata Duch.). Agrointek: Jurnal Teknologi Industri Pertanian 16 (4): 551-559. https://doi.org/10.21107/agrointek.v16i4.12336. [Indonesian]

Patel K, Soni A, Tripathi R. 2023. Pumpkin seed: Nutritional composition, health benefits. Intl J Home Sci 9 (3): 93-98.

Patel S. 2013. Pumpkin (Cucurbita sp.) seeds as nutraceutic: A review on status quo and scopes. Mediterr J Nutr Metab 6 (3): 183-189. https://doi.org/10.3233/s12349-013-0131-5.

Prommaban A, Kuanchoom R, Seepuan N, Chaiyana W. 2021. Evaluation of fatty acid compositions, antioxidant, and pharmacological activities of pumpkin (Cucurbita moschata) seed oil from aqueous enzymatic extraction. Plants 10 (8): 1582. https://doi.org/10.3390/plants10081582.

Qodri UL. 2024. Komposisi β-karoten pada biji labu kuning (Cucurbita moschata (Duch.) Poir.) menggunakan variasi pelarut. Jurnal Sains dan Teknologi 5 (3): 991-994. https://doi.org/10.55338/saintek.v5i3.2778. [Indonesian]

Rif’an, Nurrahman, Aminah S. 2017. Pengaruh jenis alat pengering terhadap karakteristik fisik, kimia dan organoleptik sup labu kuning instan. Jurnal Pangan dan Gizi 7 (2): 104-116. https://doi.org/10.26714/jpg.7.2.2017.104-116. [Indonesian]

Singh A, Kumar V. 2021. Nutritional, phytochemical, and antimicrobial attributes of seeds and kernels of different pumpkin cultivars. Food Front 3 (1): 182-193. https://doi.org/10.1002/fft2.117.

Vinayashree S, Vasu P. 2021. Biochemical, nutritional and functional properties of protein isolate and fractions from pumpkin (Cucurbita moschata var. Kashi Harit) seeds. Food Chem 340: 128177. https://doi.org/10.1016/j.foodchem.2020.128177.

Xie Y, Wang Y, Jin X, Zhang X, Yang R. 2025. Pumpkin seed proteins: The potentially alternative protein supplements for food applications. Foods 14 (22):3969. https://doi.org/10.3390/foods14223969.